MCF7/S0.5 was adapted to grow in low-serum culture medium to reduce estrogen exposure from fetal bovine serum. This permits the cells to be used for experiments studying hormone signaling and the effects of drugs that target hormone receptors. This line was used as the parental line from which the tamoxifen-resistant and fulvestrant-resistant MCF7 cell lines were generated. Therefore, drug sensitive and resistant matched cell lines are available.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
|Donor age at sampling||69 yrs|
|Size||1 million cells|
|Disease information||Malignant adenocarcinoma derived from pleural effusion|
|General notes||Expresses the estrogen receptor (ER)|
|Shipping conditions||Dry ice|
|Storage conditions||Liquid nitrogen|
|Usage notes||Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin|
|Condition of sale||The end user is not permitted to transfer or re-freeze the cells|